Phoebe,
my understanding is that power of UV illumination here is not in
salt-vs-protein test (which, imho, can only be finalized by testing
diffraction) but in improved contrast for protein crystal detection. I
haven't used UV microscopes myself, but images provided by manufacturers
are quite impressive
http://www.rigaku.com/images/automation/uv-before.jpg
http://www.rigaku.com/images/automation/uv-after.jpg
Of course, these images are advertisement and may be exaggerated.
On the salt/protein issue, I wholeheartedly agree.
Ed.
On Wed, 2010-01-20 at 13:52 -0600, Phoebe Rice wrote:
> Are these things really cost-effective? That is, compared to
> the cost of simply posting a list of evil salts like Mg
> ammonium phosphate on the wall and testing a few duds in the
> x-ray beam?
> Phoebe
>
> ---- Original message ----
> >Date: Wed, 20 Jan 2010 13:59:23 -0500
> >From: Gabriel Birrane <[log in to unmask]>
> >Subject: Re: [ccp4bb] UV microscope for screening
> >To: [log in to unmask]
> >
> >I received this in the mail today. You may be interested.
> >
> >http://www.fmpproducts.com
> >
> >They have products for crystallography
> >
> >
> >On Wed, 2010-01-20 at 11:28 -0500, Scott Walsh wrote:
> >> Dear CCP4BB members,
> >>
> >> I am shopping around for a UV microscope for the lab and
> would like to get some feedback
> >> about peoples' experiences using UV microscopes for
> screening crystals.
> >>
> >> Best,
> >>
> >> Scott
> >>
> >> *********************************************************
> >> Scott T. R. Walsh, Ph.D.
> >> Assistant Professor
> >> Center for Advanced Research in Biotechnology
> >> University of Maryland Biotechnology Institute
> >> Rm 3127E CARB II
> >> 9600 Gudelsky Drive
> >> Rockville, MD 20850
> >> email: [log in to unmask]
> >> phone: (240) 314-6478
> >> fax: (240) 314-6255-
> >>
> Phoebe A. Rice
> Assoc. Prof., Dept. of Biochemistry & Molecular Biology
> The University of Chicago
> phone 773 834 1723
> http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
>
> RNA is really nifty
> DNA is over fifty
> We have put them
> both in one book
> Please do take a
> really good look
> http://www.rsc.org/shop/books/2008/9780854042722.asp
--
Edwin Pozharski, PhD, Assistant Professor
University of Maryland, Baltimore
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When the Way is forgotten duty and justice appear;
Then knowledge and wisdom are born along with hypocrisy.
When harmonious relationships dissolve then respect and devotion arise;
When a nation falls to chaos then loyalty and patriotism are born.
------------------------------ / Lao Tse /
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