Dear Muhammed,
In case you have used phaser for MR you could try to either decrease
sequence identity or increase the rmsd of the search model (both ways
are equivalent). This allows also to account for flexibility/movements
in your target protein like Tim explained.
Cheers,
christian
Muhammed bashir Khan wrote:
> Dear All;
>
> We have solved a crystal structrure of protein at 1.8 A. I have now
> another crystal of the same protein in aother unit cell,for the new
> crystal type resolution is 3.6 A but when I use our structure as a seach
> model It does't give any solution.
>
> Any suggestion would be highly appreciated.
>
>
_______________________________________________________________________
Dr. Christian Biertümpfel
Laboratory of Molecular Biology
NIDDK/National Institutes of Health phone: +1 301 402 4647
9000 Rockville Pike, Bldg. 5, Rm. B1-03 fax: +1 301 496 0201
Bethesda, MD 20892-0580
USA
_______________________________________________________________________
|