Hello Vin,
have you tried fitting two glycerol molecules with complementary
occupancies?
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Fri, 8 Jan 2010, Vin Purp wrote:
> Can a trained eye identify this blob? This is 1.65 Ang resolution. There are
> very few components in the M.L. and protein purification buffers:
>
> ~1.5 ammonium sulfate, ~50 mM TRIS-HCl buffer pH 8.5, ~5 mM
> 2-mercaptoethanol, and ~25% glycerol. That's it.
>
>
> Glycerol fits the 'forked' portion, but does not account for the opposite
> end that looks like a carboxylate. Are there any common contaminants
> associated with glycerol (from aging), or left overs from synthesis (dirty
> batch)? Any help is appreciated.
>
> V
>
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