Hi Martyn,
When it comes to micro-dialysis, you could check the following paper:
http://www.jbc.org/content/260/25/13580.full.pdf+html
(freely available, check in particular the appendix for the
micro-dialysis setup that was used, that was for heat-labile enterotoxin)
Fred.
PS I know this is not exactly answering your question but still you
might find this setup useful
MARTYN SYMMONS wrote:
> Thanks to those who replied.
>
> The humour comes of course from a slight feeling that there is
> some truth in the 'respectful' version. Proteins are wonderful subtle
> machines so perhaps we should be careful and thoughtful when we expose them to the
> horrors of most crystallization conditions!
>
> Which takes me to the original point of my search - which was for a dialysis method for lysozyme. I was trying to set up a micro dialysis format for my protein but wanted to test it first. I am using spectrapore 8K dialysis membrane and wondered if that would retain lysozyme enough to get crystals (I'd like to stick with this cut-off as the physical and sealing properties of other membranes might be different). Or if someone has a higher MW cheap alternative protein/condition?
>
> Best wishes - agus 'Bliadhna Mhath Ur Dhuibh Uile' as we say in gaelic.
>
> Martyn
> -----
> Martyn Symmons
> Cambridge
>
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