Hi Martyn,
this recipe tends to work for me...
Lysozyme: 50 mg/ml in 0.1 M Sodium Acetate pH 4.8
Reagent: 8% w/v Sodium Chloride, 0.1 M Sodium Acetate pH 4.8
Additional Reagents: Index Reagent 8, 22, 28, 31, 34, 40, 58, 59, 69, 86, 88
Mix equal amounts of lysozyme with reagent, incubate at 4 or 22
degrees Celsius. Batch or vapor diffusion works fine.
==> Direct copy/paste from http://hamptonresearch.com/experiments.aspx <==
HTH and Happy Christmas.
Cheers,
Dave
============================
David C. Briggs PhD
============================
University of Manchester E-mail:
[log in to unmask]
============================
Twitter: @xtaldave
Skype: DocDCB
============================
2009/12/21 MARTYN SYMMONS <[log in to unmask]>:
> Dear All
> checking out the Lysozyme crystallization methods on the web I liked the Rigaku Instructions that I found:
> (http://www.rigaku.com/protein/crystallization.html)
>
> "...create a drop of 3ul lysozyme solution, and 3 ul of well solution, respectfully, for a total drop size of 6ul..."
>
> So perhaps sometimes I am just not respectful enough to deserve crystals ?
>
> good wishes to all
> regards,
> Martyn
> -----------------------
> Martyn Symmons
> MRC-MBU Cambridge UK
> 'Chan fhiosrach mur feňraich.'
> Gaelic proverb -
> Nothing asked, nothing learned.
>
|