Dear all,
I have a crystal that can diffract to 3.0A for the first few frames at
synchrotron, after procession only up to 3.6A data are useful. The
structure has been solved by MAD. In order to get a 3.0A dataset, I am
going to collect the first few images from dozens of isomorphous
crystals and merge them together. Because i have no experience like this
before, i am here asking for some help or suggestions. Especially I have
some specific questions:
1. Is it possible to extend the resolution of the structure to 3.0A by
this method?
2. At least how many frames should i collect for a single crystal for
data procession?
3. Since the crystal can grow to a large dimension (0.3x0.3x0.4mm), can
i collect the dataset at different positions of the crystal so that the
a single crystal can be re-used for several times thus to reduce the
number of total crystals needed.
4. How many frames in total should be enough for final data procession
and scale.
5. which programs(hkl, xds, ...) are the best for merging the dataset.
6. is there anything i need to pay particular attention to during data
collection and procession.
7. Some tricks, tips or references for such a job.
I appreciate any responses.
Best Regards,
lithin
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