What is the space group? Is there unmodeled density? Are all residues
in your construct built? What is the refinement protocol?
FR
On Dec 18, 2009, at 6:47 AM, james09 pruza <[log in to unmask]>
wrote:
> Dear All,
>
> I am trying to solve a 2.55 A resolution data set. The R-factor is
> around 24% while Free-R is 30%. The Residues in most favourable
> region is 95% while additionally allowed region is 5%. What are the
> ways to reduce the R-factor and Free-R? Around 100 water molecules
> are placed.
>
> Thanks in advance for the help.
>
> J...
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