Dear all,
I have applied the cluster-based thresholding technique in TBSS to analyse my
DTI data. The threshold was set to t > 1, which leads basically to one
significant cluster covering all white matter tracts. A higher threshold level
gave similar results (note we are using a preclinical mouse model of severe
hypomyelination), ie covering all prominent tracts.
1st question:
Is there any concern setting the threshold level so low?
(I wouldn't think so as the cluster size is being tested against a critical size)
2nd question:
As the final cluster covers all most prominent white matter tracts (without any
disconnection), is it legitimate to say that, for instance, the anterior
commissure, corpus callosum and so forth are significantly altered, or what
could be a more accurate wording?
Thanks for any hints :)
All the best,
Torsten
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