Dear Colleagues,
In the proper buffer (e.g. PBS) and after sterile filtration
(possibly, also upon addition of 0.02 % NaN3) Fab fragments can be
stored at 4 oC and stay functional over more than a year.
For long term storage they should be shock-frozen in liquid nitrogen
and may be kept at -20 or -80 oC. As for any protein, repeated
freeze-thawing should be avoided.
Arne Skerra
At 4:02 Uhr -0500 13.11.2009, Anna Gardberg wrote:
>Hello Jan,
>I stored intact IgGs as an ammonium sulfate cut at 4C with a little
>azide for several months with no problems, but I can't recall ever
>trying long-term storage of the Fabs. Good luck.
>
>-Anna
>
>On Fri, Nov 6, 2009 at 8:52 AM, Jan Rash <[log in to unmask]> wrote:
>> Dear All,
>>
>> Does any body have any clue about the stability of monoclonal Fab fragments.
>> How long can they survive at 4 degree? What is an ideal temperature for
>> their storage; 4 degree, -20 or -80.
>>
>> thanks a lot
>>
> > Jan
--
------------------------------------------------------------------------
Prof. Dr. Arne Skerra [log in to unmask]
Lehrstuhl f. Biologische Chemie Phone: +49 (0)8161 71-4351
Technische Universitaet Muenchen Fax: -4352
85350 Freising-Weihenstephan
Germany http://www.wzw.tum.de/bc
------------------------------------------------------------------------
|