Hi Jan,
We have tried crystallizing a similar protein without success, later on
it turned out that the protein was having a strong interaction with DNA
that was not sequence specific. You might have a case like that....
Flip
Jan Rash wrote:
> Dear All,
>
> I have a question regarding the crystallization of lysine and arginine
> rich protein around 13%. So far our attempts to crystallize this protein
> have not been successful although the secondary structure predictions,
> CD spectroscopy measurements clearly show that this protein is folded. I
> presume that these lysine and arginine are the sources of the local
> flexibility in the protein even though the protein is globular overall.
> Moreover, my attempts to crystallize the limited proteolysis fragments
> also did not achieve crystals. I have also tried the crystallization
> with its binding partners and could not succeed. I think any compound
> that binds to the lysine/arginine side chains might affect the
> crystallization process thereby reducing the internal flexibility of
> protein. Can anybody suggest some effective strategy for the
> crystallization?
>
>
>
> Thanks
>
> Umar
>
>
>
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