On 13:16 Wed 11 Nov , Matthias Zebisch wrote:
> What is the optimal wavelength for Sulfur SAD phasing?
> Is it 1.9A or should one go below that to reduce absorption/damage.
Some additional advice about factors other than wavelength: For S-SAD on
a home Cu source, my previous lab showed that optimizing (NOT
maximizing!) redundancy and using *lower* resolution were important. The
reference:
G. N. Sarma and P. A. Karplus. "In-house sulfur SAD phasing: a case
study of the effects of data quality and resolution cutoffs." Acta
Cryst. (2006). D62, 707-716.
--
Thanks,
Donnie
Donald S. Berkholz, Postdoctoral research fellow
James R. Thompson lab, Physiology & Biomedical Engineering
Grazia Isaya lab, Pediatric & Adolescent Medicine
Mayo Clinic College of Medicine
200 First Street SW
Rochester, MN 55905
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