Hello,
The situation I'm looking at may be rather complicated but I'll try to explain it. I hope you can manage to understand it.
Recently I set up Chain B and copied existing assignments of Chain A to transfer as this is homodimer protein. I did this just for one experiment so I see eg. (A/B)15ThrH(A/B)15ThrN(A/B)14SerHa on that spectrum while others show only assignments for the Chain A. In the Assignment:Assignment Panel popup, I can see assignments for Chain A and B.
Now I have a peak of which one dimension has not been assigned. I have lots of options in the right hand side table of the Assignment Panel popup but can only find the right option of the Chain A. I scrolled down or ticked Double Tolerances but still cannot find that atom from the Chain B. So I clicked <New> and tried to assign this new atom (a number [XXX] appears in the left hand side table). However, looking at the Molecules:Atom Browser popup, this atom has already been assigned (colour has been changed in the table). At this point, if I click this atom and click 'Show peaks', Peaks:selected Peaks popup appears but it's empty.
If I try to assign this forcibly, I've got a Query 'Resonance already present for atom selection. The existing atom-assigned resonance was used for this peak dimension. Merge resonance [XXX] with the one for YYY?' Then I click 'No', and this assignment appears both in the right hand side and the left hand side tables, but [XXX] remains in the right hand side table. (If I go back to Atom Browser here and choose the atom, click 'Show peaks', now I have one line in Peaks:Selected Peaks popup. (Probably a separate issue but this Show Peaks ->Selected Peaks popup is not working well, either. If I use this function, and try to look another atom it doesn't refresh or update but still show the previous ones. even if I close and reopen the popup.) I think this behaviour is strange. Could it be a bug?
If I look Atom Browser carefully and compare Chain A and B, some extra atoms appeared to be assigned just for the Chain B (the colour is different in the table) while the same atom of the chain A has not been, which seems to be strange as Chain B is copy of Chain A and I haven't assigned atoms of Chain B alone since I created it. I selected this atom and clicked 'Show peaks' button, and the Selected Peaks popup is empty.
I worry if I muddled analysis by setting up the new chain. Do you have any idea? I appreciate any suggestion. Thank you.
Kaeko
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