I've had a run of good luck with magic triangle
(5-amino-2,4,6-triiodoisophthalic acid or I3C). Its inexpensive if
you buy the powdered form and make it yourself. And you can
quickly tell if you have the correct structure solution because
the iodines are arranged in equilateral triangles approximately 6
angstroms apart. It also works at room temperature in case you
don't have a cryo system in place. Tobias Beck just recently
published a how-to in Acta (Acta Cryst F65 (10):1068-70).
Good Luck,
Katherine
On Mon Oct 26 16:44:17 EDT 2009, Hong Zhang <[log in to unmask]>
wrote:
> Xe is also an excellent anomalous scatterer at Cu Ka wavelength.
> It is
> particularly good for SIRAS phasing since Xe derivative is
> usually highly
> isomorphous to the native crystals.
>
> Hong
>
>
> _____________________
> Hong Zhang, Ph.D.
> Department of Biochemistry
> University of Texas Southwestern Medical Center
> 5323 Harry Hines Blvd.
> Dallas, Texas 75390-8816
> Phone: 214-645-6372
> Fax: 214-645-5948
> Web: http://hhmi.swmed.edu/Labs/hz/
> -----Original Message-----
> From: CCP4 bulletin board [mailto:[log in to unmask]] On
> Behalf Of Georg
> Zocher
> Sent: Monday, October 26, 2009 3:31 PM
> To: [log in to unmask]
> Subject: Re: [ccp4bb] SAD phasing at home source
>
> Dear James,
>
> A little bit more "old school" but you might also try Uranium,
> Samarium and Gadolinium.
> In principle, take a look what compounds you have in your HA box
> and afterwards check anomalous signal for example at
>
> http://skuld.bmsc.washington.edu/scatter/AS_form.html
>
> Best regards,
> Georg
>
>
>
> james09 pruza schrieb:
>> Dear All,
>>
>> I need some suggestions regarding the SAD phasing using home
>> source. What the the most commonly used HA for SAD phasing for
>> Cu anode.
>> All suggestions are welcome.
>> Thanks.
>>
>> James
>
>
> -- Universit??t T??bingen
> Interfakult??res Institut f??r Biochemie
> Dr. Georg Zocher
> Hoppe-Seyler-Str. 4
> 72076 Tuebingen
> Germany
> Fon: +49(0)-7071-2973374
> Mail: [log in to unmask]
> http://www.ifib.uni-tuebingen.de
>
>
--
SIPPEL,KATHERINE H
Ph. D. candidate
Department of Biochemistry and Molecular Biology
College of Medicine
University of Florida
|