Dear Crystallographers,
I have just returned from a seminar in which Robert Sauer said that they saw
a change in cell volume by 25% upon soaking crystals with substrate
(ATP-gammaS). He also showed that one of the cell constants changed by
about -25 Ang from 200 Ang. I am assuming that the space group did not
change--otherwise this phenomenon would not deserve comment. I asked whether
he could see the crystals change size under the microscope, and he said a
hard-to-interpret "yes."
Has anybody seen such large changes in cell constants in soaks, without
cracking? If so, could you actually see the crystals shrink as the ligand
entered the crystal?
Regards,
Jacob Keller
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [log in to unmask]
*******************************************
|