Subject: | | Re: taming structural biology |
From: | | Eleanor Dodson <[log in to unmask]> |
Reply-To: | | [log in to unmask][log in to unmask], 6 Sep 2009 12:21:27 -0400402_ISO-8859-1 Lower the concentration did help a little bit. I didn't the 6 mg/ml setups, but still got some skins. You are right the skins are more likely to find in the condition of PEGs...but also find in other conditions. Lower the conc. of PEGs is a good advice, and I'll try it. Thanks. Shu
On Sun, Sep 6, 2009 at 10:29 AM, Jeremiah Farelli <[log in to unmask]> wrote: [...]40_6Sep200912:21:[log in to unmask] |
Date: | | Mon, 28 Sep 2009 15:04:19 +0100 |
Content-Type: | | text/plain |
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I like the David Blow book very much
Outline of Crystallography for Biologists /by David Blow
<http://search.barnesandnoble.com/booksearch/results.asp?ATH=David+Blow>/
And there is another good one by Jan Drenth..
Eleano
Katja Schleider wrote:
> Hello everyone,
>
> I am no structural biologist but a microbiologist. I have a cooperation with a crystallographic group to solve the structure of a bacterial enzyme. Now we have recorded a dataset and are solving the structure. My problem is that the whole Structural Biologist thing is so complex (Biology, chemistry physics...). Nevertheless its very fascinating. But what I want to know is, if there is a good textbook for it. I got "crytallography made crystal clear" but this book isn't enough at all. Especially for data processing. I want to know things like which values are good for Rfactor observerd/expected or for R-meas Rmrgd-F after the correctstep with xds. Is 80% completness still ok? And after scaling we had a Rmerge of 0.156 (overall; at 2.2 A).
> My pHD-cooperation partner isn't that experienced as well, so its difficult to understand everything and do everything correct.
> I hope these questions are ok and somebody can help with a textbook or publication.
>
> Thanks in advance,
>
> Katja
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