Chris
Although generally less popular nowadays, microbatch-under-oil has great
advantages for anaerobic work. You can keep your stock solutions in the
chamber all the time, so you only need to degas your protein. Microbatch
finds roughly as many crystals in screening experiments as vapor
diffusion (summary at http://www.douglas.co.uk/mbnvdall.htm ). Of
course the oil helps to protect the protein too - generally less is
denatured on the surface
For an automatic setup that fits in an anaerobic chamber conveniently
see the winning entry in Douglas Instrument's competition (2006), from
the University of Georgia, Athens
http://www.douglas.co.uk/presentations/winner2_files/v3_document.htm
Hope that's useful
Patrick
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> -----Original Message-----
> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
> Christopher Rife
> Sent: 02 September 2009 18:34
> To: [log in to unmask]
> Subject: [ccp4bb] anaerobic crystallization
>
> Hi,
>
> Does anyone have tips or suggestions for getting started with
anaerobic
> crystallization? Searching through the archives, I was able to find
> some
> discussion about various glove box options
> (http://tinyurl.com/ccp4-glovebox), but not about the process itself
> (we
> already have a box).
>
> To simplify things, would it be worthwhile to perform the initial
> screens
> in something like the pre-filled Qiagen screens
> (http://tinyurl.com/qiagen-xseal)? Do I need to worry about
evaporation
> while solutions are being degassed (esp volatiles)? Better/easier to
> try
> microbatch?
>
> Thanks for any input.
>
> Chris
>
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