A little tangental.... You mentioned lowering the temperature - how
low... Stratagene markets a call line - Arctic Express - that adds
chaperones that are more active at lower temps. I know someone who
overcame inclusion body problems by expression at 16 using these cells.
(I know someone else who regularly induces o/n at 16C w/ regular
cells). The only draw back is that these cells produce a protein that
binds to NiNTA resin...
Good luck...
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