Hello All,
I dug around on the net and found this method to remove lipids from
proteins:
"Wessel & Fluegge (1984), Anal. Biochem. 138:141-143. Itīs a methanol/
chloroform precipitation and gives you a pellet that is easily
redissolved. The method was especially devised for removing lipids or
detergents, so it should be perfect for you."
-- http://www.bio.net/bionet/mm/methods/1996-December/052513.html
Is this still the preferred way? I do not want to use reagents that
are *themselves* likely to denature my protein. Has anyone tried
cyclodextrins? I'm specifically trying to strip sarcosyl. I want to do
it completely.
James
|