I second that one. Ammonium sulfate is one of my favorite cryos. I
recommend making up a saturated solution of ammonium sulfate, as it is
actually a very good cryo all by itself, and then "dilute" it by adding
the rest of the stuff in your condition (buffers, etc. and a little bit
of water). You want to be a little below saturation so that the salt
does not grow crystals of its own while you are soaking. This is
especially important if your protein crystals are hexagonal rods!
-James Holton
MAD Scientist
Savvas Savvides wrote:
> Hi Brenda
> Try > 3M ammonium sulfate itself! We have tried that with great succes by
> cryo-cooling xtals grown at 3.2M AS straight out of their crystallization
> drops. You can consult the "M&M" section in Kyndt J. et al Biochemistry 2007
> Jan 9;46(1):95-105 for a more detailed description of what we did.
>
> Best of luck
> Savvas
>
> ----
> Savvas Savvides
> L-ProBE, Unit for Structural Biology
> Ghent University
> K.L. Ledeganckstraat 35
> 9000 Ghent, BELGIUM
> office: +32-(0)9-264.51.24 ; mobile: +32-(0)472-92.85.19
> Email: [log in to unmask]
> http://www.lprobe.ugent.be/xray.html
>
>
>
> -----Original Message-----
> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
> Schulman, Brenda
> Sent: Wednesday, August 19, 2009 6:19 PM
> To: [log in to unmask]
> Subject: [ccp4bb] Cryoprotection in > 3M ammonium sulfate
>
> Hello!
>
> I would be grateful for suggestions on cryoprotectants for crystals growing
> in > 3M ammonium sulfate.
>
> Thanks!
>
> Brenda
>
>
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