Or saturated LiSO4 works too. Should work in this case.
-Tom
Tom J. Brett, PhD
Assistant Professor of Medicine
Division of Pulmonary and Critical Care
Washington University School of Medicine
Campus Box 8052, 660 S. Euclid
Saint Louis, MO 63110
________________________________________
From: CCP4 bulletin board [[log in to unmask]] On Behalf Of Savvas Savvides [[log in to unmask]]
Sent: Wednesday, August 19, 2009 11:47 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] Cryoprotection in > 3M ammonium sulfate
Hi Brenda
Try > 3M ammonium sulfate itself! We have tried that with great succes by
cryo-cooling xtals grown at 3.2M AS straight out of their crystallization
drops. You can consult the "M&M" section in Kyndt J. et al Biochemistry 2007
Jan 9;46(1):95-105 for a more detailed description of what we did.
Best of luck
Savvas
----
Savvas Savvides
L-ProBE, Unit for Structural Biology
Ghent University
K.L. Ledeganckstraat 35
9000 Ghent, BELGIUM
office: +32-(0)9-264.51.24 ; mobile: +32-(0)472-92.85.19
Email: [log in to unmask]
http://www.lprobe.ugent.be/xray.html
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Schulman, Brenda
Sent: Wednesday, August 19, 2009 6:19 PM
To: [log in to unmask]
Subject: [ccp4bb] Cryoprotection in > 3M ammonium sulfate
Hello!
I would be grateful for suggestions on cryoprotectants for crystals growing
in > 3M ammonium sulfate.
Thanks!
Brenda
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