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CCP4BB  August 2009

CCP4BB August 2009

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Subject:

Re: imosflm with multiple data sets

From:

hari jayaram <[log in to unmask]>

Reply-To:

hari jayaram <[log in to unmask]>

Date:

Mon, 17 Aug 2009 09:53:37 -0400

Content-Type:

text/plain

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I didnt realize the following:

You read in images from the two wedges  collected with the same
crystal orientation.

mydata_1_###.img
mydata_101_###.img


Now when you index ,if you say use images from both "datasets"
mydata_1_###.img use image 1,90
mydata_101_###.img use image 30 , 120

The matrix for the second wedge (mydata_101_###.img)  is still marked unknown?
Isnt this different from the behaviour in the X- mosflm . SHould the
matrixes be the same since the orientation was calculated using images
from both.

Now , If I did not force the second wedge to have the same matrix ,
using the save to file and read from file method you just described ,
does the new imosflm use the last calculated matrix from the running
session or calculate a new matrix ?..I guess I have to check some of
the data I processed with my erroneous assumption to make sure that
the matrixes for the two wedges are the same .

Thanks for clarifying this..
hari




On Mon, Aug 17, 2009 at 9:13 AM, Andrew Leslie<[log in to unmask]> wrote:
> Dear Tom,
>
>                  There is a straightforward way to do what you want. It is
> probably simplest to start by reading in only the images from the first
> segment (0-180). Then do the indexing, cell refinement and integration in
> the usual way.
>
> Then read in the second segment of data. You will notice that in this second
> segment, underneath the Sector name, there is a line starting "Matrix" and
> this will be "Unknown". If you go to the Matrix line of the first segment,
> the matrix will have a name (based on the image template).  Double click on
> the name of the matrix. A popup window (Matrix properties) will appear.
> Click on the "save matrix file" icon (a blue disc) and save the matrix with
> an appropriate filename.
>
> Now go to the Matrix line of the second segment, double click (on Unknown)
> as before and this time click on the "Open matrix file" icon (a folder) and
> read in the matrix that you saved from the first sector. You can now process
> the second segment using this matrix.
>
> It would be even nicer if you could "drag and drop" the matrix, this is on
> our "to do" list.
>
> Best wishes,
>
> Andrew
>
> On 17 Aug 2009, at 13:33, Brett, Thomas wrote:
>
>> I am an imosflm novice and have a relatively simple question. I have a 360
>> deg data set collected in two swathes of 180 deg (one with phi=0 and omega
>> going 0-180 and the second with phi = 180 and omega going 0-180). What is
>> the easiest way to process the two datasets using a matching orientation
>> matrix (or one rotated by 180 deg as it were) so that all the data can be
>> merged together. Is there an easy way to do it in imosflm or must one
>> process the two sets separately and then manipulate later with pointless
>> before scalling and merging everything together?
>> Thanks in advance.
>> -Tom
>>
>> Tom J. Brett, PhD
>> Assistant Professor of Medicine
>> Division of Pulmonary and Critical Care
>> Washington University School of Medicine
>> Campus Box 8052, 660 S. Euclid
>> Saint Louis, MO 63110
>

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