The paper Acta Cryst. D59 (2003) 2125-2132 discusses searching for
disulfides. In general this works for data truncated for phasing to
between 2.1 and 2.9 A. If the resoltuions is higher than 2.1, you
can search for individual sulfurs, if it is worse than 2.9 even
fitting them as disulfides is unreliable and they have to be
treated as 'super-sulfurs'. The main advantage of incorporating
disulfides in this resolution range is that the resulting
experimental phases extend better to higher resolution.
George
Prof. George M. Sheldrick FRS
Dept. Structural Chemistry,
University of Goettingen,
Tammannstr. 4,
D37077 Goettingen, Germany
Tel. +49-551-39-3021 or -3068
Fax. +49-551-39-22582
On Mon, 10 Aug 2009, Francis E Reyes wrote:
> Hi all
>
> Has there been any work/reports of using disulfide restraints (number of heavy
> atoms as well as distance) for heavy atom searching/scoring for anomalous
> sulfur phasing?
>
> What resolution range would this be most effective?
>
> Thanks
>
> FR
>
>
> ---------------------------------------------
> Francis Reyes M.Sc.
> 215 UCB
> University of Colorado at Boulder
>
> gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
>
> 8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
>
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