Peter Grey wrote:
> Hi everyone,
>
> I am trying to use density modification at rather low resolution (4-5A )
> for an RNA structure. My first time ever with RNA.
> I usually use Histogram matching as part of the density modification
> scheme in DM. But this method is based on density distribution of
> protein maps I think.
> Is histogram matching still valid when it comes to RNA or protein/RNA
> structures ?
I have the same question with respect to metalloproteins.
Presumably the heavier metal atoms make spikes that are completely off the
scale of a normal protein histogram. Is it then a bad idea to use
histogram matching? Do the metals get flattened down to the highest
density expected for protein on every cycle?
Ed
|