Also MALDI-TOF can be used to help identify the lipid (with standards).
I vaguely remember one would extract the lipid, from the protein, using
organic solvents then apply that to the MS. When I did it (about 10
years ago) I used non plastic vials and a Hamilton syringe so as not to
contaminate the sample with plastics.
Hope that's useful!
Gina
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Soisson, Stephen
Sent: Tuesday, July 07, 2009 2:01 PM
To:
Subject: Re: [ccp4bb] Lipid content/removal
Activated charcoal (see serum albumin structure references) can remove
fatty acids.
Good luck-
Steve
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Jacob Keller
Sent: Tuesday, July 07, 2009 1:01 PM
To: [log in to unmask]
Subject: [ccp4bb] Lipid content/removal
Dear Crystallographers,
does anybody know of a simple, fast way to determine whether a protein
sample contains lipid, and roughly to determine the quantity? As
corollary,
does anybody know of a quick way to extract/remove the putative lipid?
In my
case, I am working with an extremely robust/stable soluble protein which
may
contain bacterial lipids.
Thanks for your consideration,
Jacob Keller
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [log in to unmask]
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