Hi All
I am hoping that someone could give me some advice when working with DNA
within Analysis.
I have a 2D NOESY spectrum that I am using to assign a piece of double
stranded DNA. I have entered 2 separate molecule systems (2 different
chains) for each of the strands (is there a better way of telling Analysis
its double stranded? or should I just put in one continuous sequence and
ignore its double stranded at this point?)
Now when I assign the NOESY xpeaks its getting very complicated as the
labelling doesn't distinguish between MS1 and MS2. As I have 2Gua in both
MS1 and MS2 I can foresee problems later on trying to assign NOEs when I
see two options but without knowing easily which is from MS1 and which
from MS2. Any ideas of how to get around this (other than changing to a
single molecular system with the sequence continuous)?
Another issue arising is that because the NOESY was run with high
resolution I am seeing some J coupling splitting of the NOESY peaks.
Currently I am peak picking both and then Peak -> Make Intermediate Peak
and then deleting the two original peaks - is this the right or best way?
many thanks for any help
Richard
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