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FSL  May 2009

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Subject:

Re: 2 factor repeated measures ANOVA and contrasts

From:

StŽéphane Jacobs <[log in to unmask]>

Reply-To:

FSL - FMRIB's Software Library <[log in to unmask]>

Date:

Tue, 12 May 2009 14:01:41 +0200

Content-Type:

text/plain

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text/plain (134 lines)

Hello,

Has anyone anything to advise on this? I've looked at it as well and 
can't seem to figure out how to go about it... I have no problem when 
the 2 factors have 2 levels each, but not when a factor has 4 levels...

Best,

Stéphane

Stéphane Jacobs - Chercheur post-doctorant / Post-doctoral researcher

Espace et Action - Inserm U864
16 avenue du Doyen Lépine
69676 Bron Cedex, France
Téléphone / Phone: (+33) (0)4-72-91-34-33



Kristen Macuga a écrit :
> Hi,
>
> I'd like to run a 2x4 repeated measures ANOVA and test specific contrasts within this 
> design, but I can't seem to find any examples of how to code 2 factors when one has >2 
> levels or of repeated measures designs with >1 factor.
>
> Here are the details of my design:
> -Both factors are repeated measures, such that every subject performs every condition.
> In other words, Factor A has 2 levels and Factor B has 4 levels, so each subject performs 
> A1B1 A1B2 A1B3 A1B4 A2B1 A2B2 A2B3 A2B4.
> -Each subject does 4 runs, though all conditions are included in each run.
> I also have a rest condition that follows each of the experimental conditions.  
>
> Here is how I've set up my analysis:
> 1) At the 1st level, for each run, I am contrasting each condition vs. the rest condition, 
> which gives me 8 copes.  
> 2) At the 2nd level, for each subject, I combine these 8 copes across the 4 runs.
>
> Then, here are the 3rd level analyses that seem to make sense to me, though I'm new at 
> this.  
>
> 3A) Set up the design matrix with an EV for each of the 8 conditions + EVs for each 
> subject.  Here is an example with only 2 subjects included for simplicity:
> 1	0	0	0	0	0	0	0	1	0
> 0	1	0	0	0	0	0	0	1	0
> 0	0	1	0	0	0	0	0	1	0
> 0	0	0	1	0	0	0	0	1	0
> 0	0	0	0	1	0	0	0	1	0
> 0	0	0	0	0	1	0	0	1	0
> 0	0	0	0	0	0	1	0	1	0
> 0	0	0	0	0	0	0	1	1	0
> 1	0	0	0	0	0	0	0	0	1
> 0	1	0	0	0	0	0	0	0	1
> 0	0	1	0	0	0	0	0	0	1
> 0	0	0	1	0	0	0	0	0	1
> 0	0	0	0	1	0	0	0	0	1
> 0	0	0	0	0	1	0	0	0	1
> 0	0	0	0	0	0	1	0	0	1
> 0	0	0	0	0	0	0	1	0	1
> and some example contrasts:
> A1-A2	1	1	1	1	-1	-1	-1	-1	0	0	
> B1-rest	1	0	0	0	1	0	0	0	0	0	
> B2-B1	-1	1	0	0	-1	1	0	0	0	0	
>
> and the 3 F-tests:
> A1-2          1	1	1	1	-1	-1	-1	-1	0     0
> B1-4          3	-1	-1	-1	3	-1	-1	-1	0     0
> B2-4          -1	3	-1	-1	-1	3	-1	-1	0     0
> B3-4          -1	-1	3	-1	-1	-1	3	-1	0     0
> A1-2*B1-4  3	-1	-1	-1	-3	1	1	1	0     0
> A1-2*B2-4  -1	3	-1	-1	1	-3	1	1	0     0
> A1-2*B3-4  -1	-1	3	-1	1	1	-3	1	0     0
> When I run it this way, I get a rank deficient/singular matrix error.  It runs fine without 
> the subject EVs included, but then I'm not able to remove the subject mean variability 
> from the residuals. Should I perhaps be doing this at the 1st level instead and then using 
> the multi-session, multi-subject example to average across runs and subjects? If so, how 
> can I contrast each condition vs. rest beforehand, and still do this?
>
> 3B) I also attempted to code it with 7 predictors instead.  Here is the design matrix that I 
> generated for testing individual contrasts with the following coding scheme (following 
> your tripled two-group difference example): 
> A1B1 = a + b + c + d + e + f + g
> A1B2 = -a
> A1B3 = -b
> A1B4 = -c
> A2B1 = -d
> A2B2 = -e
> A2B3 = -f
> A2B4 = -g
> 1	 1	1	 1	1	 1	1
> -1	0	 0	0	 0	0	 0
> 0	 -1	0	 0	0	 0	0
> 0	0	 -1	0	 0	0	 0
> 0	 0	0	 -1	0	 0	0
> 0	0	 0	0	 -1	0	 0
> 0	 0	0	 0	0	 -1	0
> 0	0	 0	0	 0	0	 -1
> and some example contrasts: 
> A1-A2	 0	0	 0	2	 2	2	 2
> A2-A1	 0	0	 0	-2	 -2	-2	 -2
> B1-0	         1	1	 1	0	 1	1	 1
> B2-0	         -1	0	 0	0	 -1	0	 0
> B2-B1	 -2	-1	 -1	-2	 -2	-1	 -1
> B4-B3	 0	1	 -1	0	 0	1	 -1
> B2-B4	 -1	0	 1	0	 -1	0	 1
> and the design matrix I created for the F-test (1st column codes A, 2nd-4th columns code 
> B, and 5-7th columns code interactions)
> (roughly following the ANOVA: 1-factor 4-levels (Repeated Measures) example): 
> 1	-1	-1	-1	-1	-1	-1
> 1	1	0	0	1	0	0
> 1	0	1	0	0	1	0
> 1	0	0	1	0	0	1
> -1	-1	-1	-1	1	1	1
> -1	1	0	0	-1	0	0
> -1	0	1	0	0	-1	0
> -1	0	0	1	0	0	-1
> along with the F-test matrix:
> A1-2	         1	0	 0	0	 0	0	 0
> B2-1	         0	1	 0	0	 0	0	 0
> B3-1	         0	0	 1	0	 0	0	 0
> B4-1	         0	0	 0	1	 0	0	 0
> A1-2*B2-1	 0	0	 0	0	 1	0	 0
> A1-2*B3-1	 0	0	 0	0	 0	1	 0
> A1-2*B4-1	 0	0	 0	0	 0	0	 1
> For all of these, I also have N additional columns (one for each subject) in order to 
> remove the subject mean variability.
>
> Any advice or comments would be greatly appreciated.  
> Thanks! 
> Kristen
>
>
>   

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