Hi.
We are trying to prepare heavy atom derivatives of a protein in which
a surface residue was mutated to cysteine. Mass spectrometry of the
purified cysteine mutant showed an additional peak with a molecular
mass that correspond to a mercaptoethanol-bound cysteine mutant. I am
wondering whether the bound mercaptoethanol prevents the heavy atom
from binding to the cysteine residue.
Any information would be greatly appreciated.
Wataru Kagawa
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