Hi
We are currently trying to work through an ensemble of 3D backbone
assignment spectra that have been acquired with a reduced spectral width in
the N15 dimension (gain in resolution/experimental time) and several peaks
are refolded (type States).
We have a full HSQC spectrum that we have used as a reference (in order to
obtain the correct N15 shifts) and also a reduced one. We could not find a
way to move between the (un)folded peaks in the reference spectrum and the
3D spectra and also to add the 13C frequency from a 3D spectrum to a
(refolded) spin system (different N15 shifts). One way to work around would
be to use the folded HSQC spectrum as a reference and to manually correct
the N15 shifts at the end - but I am sure that there are more elegant ways
to handle this problem.
Thank you for all your time and efforts
Sincerely
Beate
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