David Cobessi wrote:
> Liew Chong Wai wrote:
>> Hi,
>>
>> Good day
>> I am currently building my structure by using COOT. My protein is a
>> tetramer protein and I have fit my protein sequence into one of the
>> monomer of the homologous model. May I know how can I replace other
>> monomer with the amended monomer??
>> Thanks
>>
>> *Chong-wai*
>>
> Hi Chong-wai,
> Several possibilities to generate the tetramer from the monomer, let
> say A.
> You can calculate the RT matrices ( in CCP4 or using LSQMAN and
> MOLEMAN2 or using O for example) to superpose the monomer A onto B, C
> and D and then apply these matrices to the amended monomer (A) in
> order to generate the tetramer.
> You can also superpose the monomer A onto B in COOT by using SSM or
> LSQ and save the new position as monomer B in COOT.... etc... and then
> you can generate 4 monomers and the tetramer. Do not forget to change
> the chain ID before refinement.
>
Blimey.
Extensions -> NCS -> Copy NCS Chain...
See Section 5.27 of the user manual for more information.
http://www.ysbl.york.ac.uk/~emsley/coot/doc/chapters/user-manual_5.html#SEC137
Paul
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