If you go to this web-page: http://www.embl-hamburg.de/ExternalInfo/Research/Sax/user_info.html there is ashort summary of the requirements for a SAXS experiment. Shortly the sample needs to be monodisperse (>90%) and you will need a range of concentrations roughly from 0.5/1.0mg/ml to 10mg/ml
Marjolein Thunnissen Phone +46-(0)46-22 24584
Associate Professor Fax +46-(0)46-22 24692
Dept of Molecular Biophysics, Lund University http://www.mbfys.lu.se
PO-Box 124 S-221 00 Lund, Sweden
Scientific coordinator I911 (Max-lab): MAD and fixed-wavelength stations
for macromolecular crystallography
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From: CCP4 bulletin board [[log in to unmask]] On Behalf Of Li Sheng [[log in to unmask]]
Sent: Thursday, April 23, 2009 1:19 PM
To: [log in to unmask]
Subject: [ccp4bb] protein purity in SAXS
Dear all,
Is there anyone with experience in small angle x-ray scattering? Could
anyone please tell me the requirements for protein sample purity and
concentration?
Thanx in advance.
Sincerely,
Li
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Email:[log in to unmask]
Institute of Biochemistry and Cell Biology
Institutes for Biological Sciences
Chinese Academy of Sciences
320 Yue-Yang Road, Shanghai 200031, China
Tel: +86-21-5492-1217
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