Hi,
to help everyone, this observation (and similar ones) could (or maybe
should) be added to others of its kind, in the CCP4 wiki at
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Conditions_prone_to_salt_crystallization
HTH,
Kay
Jacob Keller schrieb:
> Hi All,
>
> there is a condition in the JCSG+ screen (0.2M Li2SO4, 0.1M NaOAc pH
> 4.5, 50.0% v/v PEG-400) which forms heavy precipitate when made here in
> the lab. I assume that the order of addition of compounds might make a
> difference, but doesn't this suggest that there is a high probabilty for
> salt crystals under these conditions? How stable can this solution be?
> An anecdotes/advice appreciated.
>
> I assume that the PEG makes Li2SO4 fall out of solution, or perhaps it
> is the pH?
>
> Jacob Keller
>
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> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
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--
Kay Diederichs http://strucbio.biologie.uni-konstanz.de
email: [log in to unmask] Tel +49 7531 88 4049 Fax 3183
Fachbereich Biologie, Universität Konstanz, Box M647, D-78457 Konstanz
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