Hi.
Does anyone have experience using solutions containing manganese as
crystallization buffers (buffers, not screening well solutions)? The
protein that I am working requires manganese for activity, and I have read
reports of related proteins crystallizing in manganese buffers. I made a
buffer containing 3 mM manganese, that initially had a black color then
turned to deep purple, and later almost clear. After a few weeks, the
buffer turned an orange color and contains dark manganese syrup on the
bottom. Does anyone know how to prepare a manganese buffer that is stable
enough to be used as a protein buffer for crystallization screens?
Thanks,
Matt
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