If you want to get rid of mother liquid around crystal, mount it in oil.
Howerver, what is wrong with NH4SO4? If your crystal can tolerate
near-saturated NH4SO4, it is a cryoprotectant all by itself.
-James Holton
MAD Scientist
HanJie_HCT Tai wrote:
> Hi,
>
> I have a protein-DNA complex grown in 30 -40% MPD/0.2M Ammonium
> sulfate/5% PEG 400/ 0.1M Tris (8.5).
>
> I believe that 30%MPD above does not require any cryoprotectant but i
> hv a problem to loop a crystal up. The crystal always sticks with AmSO4.
>
> I tried to add 2ul up to 6ul reservoir solution into the protein drop
> (1+1 ul). Although AmSO4 was diluted, the crystal was still surrounded
> by AmSO4.
>
> The loop size is 0.1 -0.2mm, the crystal is about 0.1 mm.
>
> I tried to grow crystal without AMSO4 or 0.1M AmSO4, but no crystals
> was shown up.
>
> Any suggestion to loop up the crystal without any AMSO4 attached?
>
> Regards,
> HCT (HanJie)
>
>
>
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