Well, what do you expect the anomalous signal contributed from your 45
seleniums in a perfect world to be when the asymmetric unit contains 1300
aa? Do you think a dataset with Rmerge of ~15% is good enough to detect a
signal of even 2%? (Note: I did not do the calculation, so I just made up
the number of 2%.)
Jim
On Fri, 20 Mar 2009, Kumar wrote:
> Hello CCP4bb members,
>
> I have been trying to obtain phases for a protein which contain ~1300aa. We
> have obtained native data to a resolution of 3.3A (Space group I222 or
> I212121). But we are having tough time phasing it.
>...
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