Does your system eliminate the need for cryoprotection? Also, how do you
time the buffer-removal/freezing steps?
JPK
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [log in to unmask]
*******************************************
----- Original Message -----
From: "Watanabe Nobuhisa" <[log in to unmask]>
To: <[log in to unmask]>
Cc: "Jacob Keller" <[log in to unmask]>
Sent: Thursday, March 26, 2009 10:50 PM
Subject: Re: [ccp4bb] Crystal vacuum cleaner
>I am not sure this is the answer for your question, but please check,
> http://www.nusrc.nagoya-u.ac.jp/WatanabeLab/XtalMount/index.html
>
> We named it as a "loopless" mounting method in the paper, but now we
> leave the loop...
>
> The vacuum cleaner I have used for the method is "my mouth". But we have
> just developed a semi-automatic equipment. I hope we will be able to
> submit the manuscript soon.
>
> Nobuhisa Watanabe, PhD.
> ===
> Synchrotron Radiation Research Center
> Department of Biotechnology and Biomaterial Chemistry,
> Graduate School of Engineering
> Nagoya University
> C1-3(651) Furo-cho Chikusa-ku, Nagoya 4648603 Japan
> Email: [log in to unmask] Fax: +81-52-789-5286
>
> On 2009/03/27, at 4:43, Jacob Keller wrote:
>
>> Dear Crystallographers,
>>
>> Has anybody ever heard of mounting crystals in tiny crystal-sized
>> capillaries, such as are pulled by patch-pipet machines, or those used
>> in microfluidics? The material could be either glass or plastic, and one
>> could have some method of continuous positive or negative pressure,
>> perhaps through a hole in the crystal cap. Anyway, once safely inside
>> the tiny capilary, one could freeze it at leisure, without concern for
>> evaporation. It would really make harvesting easy--just vacuum up the
>> crystal, then plop in LiqN2/ propane as per usual. I guess it could also
>> really be done with appropriate modification of a micro-manipulator.
>>
>> Jacob
>>
>> *******************************************
>> Jacob Pearson Keller
>> Northwestern University
>> Medical Scientist Training Program
>> Dallos Laboratory
>> F. Searle 1-240
>> 2240 Campus Drive
>> Evanston IL 60208
>> lab: 847.491.2438
>> cel: 773.608.9185
>> email: [log in to unmask]
>> *******************************************
>
>
>
>
>
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