Hello,
Tim asks if you could send us a screen shot of what the [Show Resonances]
button (from the link Seq Spin Systems popup). This will show which shifts
and atom types are used for the prediction of residue i.
For the second point, the "focus width" is intended for the y-axis. Tims
says he could rename this "focus size" if absolutely required. The x-axis
widths depend on the window size and number os strips, as the ought. BTW
the focusing can be switched off.
And he doesn't think anything is wrong with the exp types/dims given that
these are triple resonance, so isotope is unique per axis.
Wayne
On Thu, 26 Feb 2009, Steffen Graether wrote:
> Hi,
>
> I'm having two problems setting up spectra for sequential CA/CB assignments.
> I have set up the experiments ( H[N[(CA|ca[Cali]}]] and H[N[co[{CA|caC}]]] )
> and Linked Peak Lists from the appropriate 15N-HSQC experiment.
>
> I have GlyAlaHis at the C-terminus, which I have been using to start the
> assignment process. One problem is that for some reason when I go to the
> plane for the His, the sequential assignment table suggest residue i could
> be an Ala, when clearly it should be residue i-1.
>
> A second problem is that the match window focuses the y-axis (13C) to 5 ppm,
> i.e. to the Link Sequential Spin Systems focus width).
>
> Both of these suggest to me that something is wrong in my experimental axis
> setup, but I can't figure it out. In Experiments/Edit Experiments/Experiment
> Types, the exp dim are 1 (1H), 2 (13C), 3 (15N), which I assume are x,y,z
> for the display windows, while the Ref Exp Dim are 1 (1H) 3 (13C) and 2
> (15N), which I think are also correct since that is the way the data was
> originally stored.
>
> Any help would be greatly appreciated!
>
> Regards,
> Steffen
>
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