Hi Ed,
A bit late on the subject ....
I have collected atomic resolution data (around 0.97A) on both bovine
and porcine phospholipase A2 crystals which at
the time of data collection were between 10-16 years old.
Crystallization setup was liquid-liquid diffusion in glass capillaries.
Differently from the other stories, here there's no degradation
involved...just rock-solid Xtals.
In the paper we stated: 'Crystals are stable in the crystallization
solution...' which I guess well reflects their behavior.
Best wishes,
Roberto
On 5 Feb 2009, at 19:11, Edward Snell wrote:
> </lurk_mode_off>
> </dumb_question_on>
>
> Dear All,
>
> I was recently trying to find references on how age may degrade a
> crystal, i.e. grow them and use them or preserve them as fresh as
> possible. I seem to remember seeing a couple of papers on this but my
> memory is fading and I have been unable to locate them. Can anyone jog
> my memory or tell me if I'm imagining things? I've found plenty on
> the
> protein prep etc. but nothing on the crystal.
>
> Thanks,
>
> Eddie.
>
>
> Edward Snell Ph.D.
> Assistant Prof. Department of Structural Biology, SUNY Buffalo,
> Hauptman-Woodward Medical Research Institute
> 700 Ellicott Street, Buffalo, NY 14203-1102
> Phone: (716) 898 8631 Fax: (716) 898 8660
> Email: [log in to unmask] Telepathy: 42.2 GHz
>
> Heisenberg was probably here! Crystallization, how quaint!
>
> </dumb_question_off>
> </lurk_mode_on>
---
Roberto Steiner
Randall Division of Cell and Molecular Biophysics
New Hunt's House
King's College London
Guy's Campus
London, SE1 1UL
Phone +44 (0)20-7848-8216
Fax +44 (0)20-7848-6435
e-mail [log in to unmask]
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