> Yingjie Peng wrote:
> ..
>> After I have solved my strucutre, I have found my target ligand bound
>> at the potential binding site. Also, I have
>> found that there are two more ligand molecules bound along the path
>> from solvent to the binding site. I think this
>> can enrich the ligand to binding site, enhancing the local
>> concentration of the ligand, thus reducing the Km of the
>> ligand.
I'm not sure if this is anything like what you see, but sometimes more
than one ligand molecule unexpectedly binds in the active site. e.g.
http://www.rcsb.org/pdb/explore.do?structureId=1GX
http://www.ncbi.nlm.nih.gov/pubmed/15102853?dopt=Abstract
Cheers,
Charlie
--
Charlie Bond
Professorial Fellow
University of Western Australia
School of Biomedical, Biomolecular and Chemical Sciences
M310
35 Stirling Highway
Crawley WA 6009
Australia
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+61 8 6488 4406
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