I am looking for a reagent (and vendor) that will irreversible put a
raher bulky substituent on a free SH group and that does not react with
free amines (or other potential reactive groups present on a protein
surface). The connection with crystallography is that it is required for
an experiment asked by a referee necessary to confirms or reject a
hypothesis that results from a crystal structure. For this experiment it
is essential that the reaction is not reversible (so no S-S bond
formation).
Remy Loris
Vrije Universiteit Brussel
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