Hi,
you could try without heating. Just vortex your sample a few times after
adding the sample buffer and then load the gel.
good luck,
Daniela
> Is your phosphate a Na-K phosphate? If it is, then you're probably getting
> a
> K salt of SDS which is considerably less soluble than Na or Li salts.
>
> Artem
>
> -----Original Message-----
> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
> Subscribe Ccp4Bb Kn L
> Sent: Wednesday, December 17, 2008 9:26 PM
> To: [log in to unmask]
> Subject: [ccp4bb] LDAO & SDS-PAGE
>
> Hello everyone...
>
> I have a protein solubilised in 1% LDAO, 50 mM Phosphate pH 7.5, 150 mM
> NaCl. When I mix it with laemmli buffer and heat at 95C, 3 mins for
> SDS-PAGE, it aggregates (as in when you mix GuHCl with SDS) and can't be
> loaded onto the gel.
>
> Does anyone know why it happens? Thanks a lot!!!
>
> Kien
>
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