Dear colleageus,
I would like to bring to your attention that as a result of a
workshop on "Diffraction Data Collection Using Synchrotron
Radiation" held last year, we have developed a tutorial for
X-ray crystallography. The tutorial consists of five experiments,
and contains all relevant information needed to reproduce the
experiments. The sources of the chemicals are given, the
procedures described in detail, the diffraction images provided
and the path to structure solution outlined.
One may use the tutorial to teach X-ray crystallography within
a practical course or in class, or interested students can work
independently with the material to learn all the steps of structure
determination by various methods.
The five experiments are:
1) S-SAD on cubic insulin
2) Br-MAD on thaumatin
3) Molecular replacement on monoclinic lysozyme
4) Solvent ion identification in tetragonal lysozyme
5) Identification of a weakly bound ligand in lysozyme
Except for experiment 2, data collection may also be performed on
home sources.
The tutorial has been described in a paper (open access) in the
most recent issue of J. Appl. Cryst.
See: http://dx.doi.org/10.1107/S0021889808032494
All material can be downloaded from either one of the two sites:
http://www.embl-hamburg.de/Xray_Tutorial/
http://www.mx.bessy.de/bessy-ws/bessy.html
In case you want to use it or have used it already, I would of
course appreciate any feedback, so that we can continue to
improve it.
With best wishes,
Manfred Weiss (on behalf of the tutorial team)
Annette Faust, Santosh Panjikar, Uwe Mueller, Venkat Parthasarathy,
Andea Schmidt & Victor Lamzin.
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* *
* Dr. Manfred S. Weiss *
* *
* Team Leader *
* *
* EMBL Hamburg Outstation Fon: +49-40-89902-170 *
* c/o DESY, Notkestr. 85 Fax: +49-40-89902-149 *
* D-22603 Hamburg Email: [log in to unmask] *
* GERMANY Web: www.embl-hamburg.de/~msweiss/ *
* *
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