>I am trying to find a method to purify/separate phosphorylated protein
>from unpshosphorylated proteins. I have two approach in my mind
> * ion-exchange
> * Fe columns, I tried this too but limited success.
Gadolinuim-charged IDA with a shallow gradient of imidazole is supposed to
separate phosphorylated proteins best. I haven't tried it myself but this
would be the first thing I'd try.
If only small quantities are required and native gel separates the species,
that's another way of doing it.
Dima
|