Hi Susannah,
Thanks a lot for your response. I am really surprised. Ten years ago,
everyone was using BSA. I'm getting old -:)
Does this mean that everyone now focuses on purifying their extract
before the amp?
I'm sure many researchers still have samples full of Humic acids and co.
Well, I'll try without any BSA and we will see.
It would actually be nice since I noticed that BSA easily gets
contaminated.
-----Original Message-----
From: Ancient DNA List [mailto:[log in to unmask]] On Behalf Of
Susannah Baldry
Sent: Wednesday, October 29, 2008 12:39 PM
To: [log in to unmask]
Subject: Re: BSA
Dear Odile,
I am not sure which protocols in the past used BSA in the PCR mix, but
(after throwing your question at my office colleagues) we agree it is
not common now - except for some commercial enzyme kits for arrays and
sequencing. In the latter case you would be following manufacturer's
protocols which would be exhaustive. If a published paper specifies the
contents of the PCR reaction it should be complete & repeatable, so you
will be safe following their protocol as described.
Good luck with your experiments,
Kind regards,
Susannah Baldry
Odile Loreille wrote:
> Hi everyone,
>
> I'm back with a second question.
> Reading the latest aDNA papers, I noticed that many PCR protocols
don't
> mention BSA anymore.
> I was wondering if you, aDNA specialists, omitted this information
because you
> believed that the presence and amount of BSA isn't worth mentioning
or if you
> truly stopped using it in your amplifications.
>
> If you want your response to be private, please simply write to
> [log in to unmask]
>
> Thank you very much
>
> Odile
>
>
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