Hi Everyone,
I need to “direct sequence” a 59bp amplicon and so far my data look awful.
I use the ABI big dye (1.5ul for a 20ul reaction), 0.5ul of dGTP, 20pmol of
primer and still 25 PCR cycles. I still need to test different dilutions of the PCR
product.
Does anyone have any experience with modified conditions that would help
decrease the background?
Any advice would be appreciated.
Cheers
Odile
Dr Odile Loreille
Senior Research Scientist
Armed Forces DNA Identification Laboratory
1413 Research blvd
Rockville, MD 20850
USA
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