Dear Odile,
I am not sure which protocols in the past used BSA in the PCR mix, but
(after throwing your question at my office colleagues) we agree it is
not common now - except for some commercial enzyme kits for arrays and
sequencing. In the latter case you would be following manufacturer's
protocols which would be exhaustive. If a published paper specifies the
contents of the PCR reaction it should be complete & repeatable, so you
will be safe following their protocol as described.
Good luck with your experiments,
Kind regards,
Susannah Baldry
Odile Loreille wrote:
> Hi everyone,
>
> I'm back with a second question.
> Reading the latest aDNA papers, I noticed that many PCR protocols don't
> mention BSA anymore.
> I was wondering if you, aDNA specialists, omitted this information because you
> believed that the presence and amount of BSA isn't worth mentioning or if you
> truly stopped using it in your amplifications.
>
> If you want your response to be private, please simply write to
> [log in to unmask]
>
> Thank you very much
>
> Odile
>
>
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