Anything that slows growth potentially could help.
In one case, I found by re-dissolving drops with showers of small
crystals I could get one or two huge ones. I just added 2 microliters
of water to the handing drop and allowed it to re-equilibrate.
Changing temperature or any physical variable that effects solubility
can help. Adding small amounts of glycerol can help, etc.
As with all aspects of crystallization, it is highly empirical.
On Sep 9, 2008, at 11:23 PM, Vincenzo Carbone wrote:
> Dear all,
>
>
>
> I was wondering if anyone had some practical advice in regards to
> increasing
> the size of a crystal. Currently my enzyme forms these rather nice
> cubic and
> very sharp <0.1mm (in 25% peg 5000, 0.1M ammonium sulphate, 0.1M
> Tris ph
> 7.0) crystals following optimisation of the pH and precipitant
> concentrations of the initial screening condition and by playing with
> various increasing levels of protein concentration 20-60mg/ml.
>
>
>
> Has anyone had success with additive screens in increasing crystal
> size and
> what were they?
>
>
>
> Any advice would be useful thanks
>
>
>
> Vince
>
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