you can do a simple wavelength scan at the synchrotron of the protein solution frozen in a loop.
Best wishes
Kornelius
On Fri, 5 Sep 2008 12:21:29 -0400
Matthew Alan Bratkowski <[log in to unmask]> wrote:
> Hello.
>
> I am working with a protein that turns a yellowish-brown color when it is
> concentrated to around 2 mg/ml or higher in a small volume (a few hundred
> uL). I was wondering if the protein bound a metal or other prosthetic
> group that would give it this color? The protein's color somewhat
> resembles iron binding proteins, but there is no peak in the 400 nm range
> that would suggest heme, and an iron sulfur cluster is not that likely
> since there are only five cysteines in the protein. Proteins with
> structures homologous to the one I am studying bind magnesium, but are not
> know to bind other metals. Any information about what this color might
> suggest about the protein or how I could analyze possible bound metals or
> prosthetic groups using only a small amount of protein would be helpful.
>
> Matt
----------------------------------------------
Kornelius Zeth
Max Planck Institute for Developmental Biology
Dept. Protein Evolution
Spemannstr. 35
72076 Tuebingen, Germany
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Tel -49 7071 601 323
Fax -49 7071 601 349
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