On 25 Sep 2008, at 14:35, Jacob Keller wrote:
> Dear Crystallographers,
>
> I remember having seen on this listserve that coomassie stain is
> horribly non-linear in intensity per protein concentration, which
> leads me to two questions:
>
> 1. Does anybody have a reference for quantitation of coomassie's
> linearity (and possibly other stains), i.e. where and how extensive
> the linear range is, and
> 2. Can anybody suggest a more linear protein gel stain?
>
Silver stain would be an option. Not quite sure how the linearity is
when using fluorescent probes e.g. SyproOrange for protein staining.
Jürgen
> Thanks,
>
> Jacob Keller
>
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
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> *******************************************
-
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch
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