T/A cloning utilizes the overhangs left by certain polymerases as cloning
handles. To lower background, the vectors for TA cloning are often designed
to contain a rare cutter which is used during ligation to constantly re-cut
the self-ligated vector, so this way only the insert ligation product is
supposed to survive.
A.
-----Original Message-----
I have heard of TOPO-TA cloning. Not sure what T/A cloning is.
|