PS. Sorry, couldn't resist. But seriously, for those new to the list:
if you want someone to spend time writing a useful answer, then you need
to spend time formulating a useful question.
Good: specific questions with sufficient background given
Bad: general questions which are best answered by (a) a textbook/manual
or (b) doing a PhD on it.
phx.
vijay srivastava wrote:
> hi
> i am facing problem in cloning,getting my insert and vector at the
> correct position after digestion but after ligation colony is not
> coming and if how it is coming than i am not getting my insert
>
>
>
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